학력 Education  

1994. 02     B.S. in Microbiology. Department of Microbiology, College of Natural Science, Seoul National University, Korea
1996. 02     M.S. in Microbiology. Department of Microbiology, College of Natural Science, Seoul National University, Korea
                    “Regulation of Paraquat-Inducible Genes by the soxRS Locus in Escherichia coli.”
                    Thesis advisor: Prof. Jung-Hye Roe, Ph.D.
2000. 08     Ph.D. in Molecular Microbiology. Schools of Biological Sciences, Seoul National University, Korea
                    “Regulation of Superoxide-Inducible Genes in Escherichia coli.”
                    Thesis advisor: Prof. Jung-Hye Roe, Ph.D.

 경력 Experience 

2000 - 2001     Post-doctoral fellow, Research Center for Molecular Microbiology,Institute of Microbiology,
                          Seoul National University, Korea. Principal investigator : Prof. Jung-Hye Roe, Ph.D.
2001 - 2002     BK21 post-doctoral fellow, School of Biological Sciences, Seoul National University, Korea.
                          Principal investigator : Prf. Jong-Sik Chun, Ph. D.
2002. 03-08     Post-doctoral fellow, Research Center for Molecular Microbiology, Institute of Microbiology,
                          Seoul National University, Korea. Principal investigator : Prof. Jung-Hye Roe, Ph.D.
2002 - 2005     Post-doctoral fellow, Research Center for Molecular Microbiology, Institute of Microbiology,
                          Seoul National University, Korea. Principal investigator : Prof. Jung-Hye Roe, Ph.D.

 2005 - 2006    Senior fellow, Department of Microbiology, University of Washington.
                          Principal investigator : Prof. E. P. Greenberg, Ph.D.
 2006 - 현재      Professor, Department of Pharmacy, College of Pharmacy,
                          Pusan National University, Republic of Korea.

 연구업적 Achievements  

Research articles
1. Soo-Kyoung Kim, Ha-Young Park, and Joon-Hee Lee*. Anthranilate deteriorates biofilm structure of Pseudomonas aeruginosa and antagonizes the biofilm-enhancing indole effect. Appl. Environ. Microbiol., in press

2. Sohee Kim, Une Teak Jung, Soo-Kyoung Kim, Joon-Hee Lee, Hak Soo Choi, Chang-Seok Kim, and Myung Yung Jeong*. Nanostructured Multifunctional Surface with Antireflective and Antimicrobial Characteristics. ACS Appl. Mater. Interfaces., 7: 326-331, 2015

3. Yong-Gyun Jung, Jungil Choi, Soo-Kyoung Kim, Joon-Hee Lee*, and Sunghoon Kwon*. Embedded biofilm: a new biofilm model based on the embedded growth of bacteria. Appl. Environ. Microbiol., 81(1): 211-219, 2015

4. Su-Jin Park, Soo-Kyoung Kim, Yong-In So, Ha-Young Park, Xi-Hui Li, Doo Hwan Yeom, Mi-Nan Lee, Bok-Luel Lee, and Joon-Hee Lee*. Protease IV, a quorum sensing-dependent protease of Pseudomonas aeruginosa modulates insect innate immunity. Molecular Microbiology, 94(6): 1298-1314, 2014

5. Jiyeun Kate Kim, Jeong Yun Kwon, Soo Kyoung Kim, Sang Heum Han, Yeo Jin Won, Joon Hee Lee, Chan-Hee Kim, Takema Fukatsu, Bok Luel Lee*. Purine Biosynthesis, Biofilm Formation, and Persistence of an Insect-Microbe Gut Symbiosis. Applied and Environmental Microbiology, 80(14): 4374-4382, 2014

6. Mi-Nan Lee, Soo-Kyoung Kim, Xi-Hui Li, and Joon-Hee Lee*. Bacterial virulence analysis using brine shrimp as infection model in relation to the importance of quorum sensing and proteases. J. Gen. Appl. Microbiol., 60:169-174, 2014

7. Doo Hwan Yeom, Soo-Jin Im, Soo-Kyoung Kim, and Joon-Hee Lee*. Activation of Multiple Transcriptional Regulators by Growth Restriction in Pseudomonas aeruginosa. Mol. Cells, 37(6): 480-486, 2014

8. Changwan Ha, Soo-Kyoung Kim, Mi-Nan Lee, and Joon-Hee Lee*. Quorum sensing-dependent metalloprotease VvpE is important in the virulence of Vibrio vulnificus to invertebrates. Microbial Pathogenesis, 71-72: 8-14, 2014

9. Soo-Kyoung Kim, Mi-Nan Lee, and Joon-Hee Lee*. Time-course Analysis of Biofilm Formation in Quorum Sensing-deficient Bacteria. Korean Journal of Microbiology, 50(2): 108-113, 2014

10. Doo Hwan Yeom, Soo-Kyoung Kim, Mi-Nan Lee and Joon-Hee Lee*. Pleiotropic effects of acyltransferases on various virulence-related phenotypes of Pseudomonas aeruginosa. Genes to Cells, 18: 682-693, 2013

11. Su-Jin Park, Hai-Bo Liu, Sunghoon Park, Joon-Hee Lee*. Modulation of QscR, a quorum sensing receptor of Pseudomonas aeruginosa, by truncation of a signal binding domain. Research in Microbiology, 164: 375-381, 2013

12. Bo-Young Yoon, Yong-Hak Kim, Nahee Kim, Bo-Young Yun, Jin-Sik Kim, Joon-Hee Lee, Hyun-Soo Cho, Kangseok Lee and Nam-Chul Ha* Structure of the periplasmic copper-binding protein CueP from Salmonella enterica serovar Typhimurium. Acta Cryst. D69, 1867-1875, 2013.

13. Soo-Kyoung Kim, Su-Jin Im, Doo-Hwan Yeom, and Joon-Hee Lee*. AntR-mediated bidirectional activation of antA and antR, anthranilate degradative genes in Pseudomonas aeruginosa. Gene, 505:146-152, 2012

14. Changwan Ha, Seong Joon Park, Su-Jin Im, Su-Jin Park, and Joon-Hee Lee*. Interspecies Signaling through QscR, a Quorum Receptor of Pseudomonas aeruginosa. Mol. Cells, 33:53-59, 2012

15. Su-Jin Park, Soo-Kyoung Kim, and Joon-Hee Lee*. Analysis of Amino Acid Residues Affecting the Activity of QscR, a Quorum Sensing Receptor of Pseudomonas aeruginosa. Korean Journal of Microbiology, 48(3):180-186, 2012

16. Junil Kang, Taeyoung Kim, Yongsug Tak, Joon-Hee Lee, and Jeyong Yoon*. Cyclic voltammetry for monitoring bacterial attachment and biofilm formation. Journal of Industrial and Engineering Chemistry, 18: 800-807, 2012

17. Yusang Choi, Ha-Young Park, Seong Joon Park, Su-Jin Park, Soo-Kyoung Kim, Changwan Ha, Su-Jin Im, and Joon-Hee Lee*. Growth Phase-Differential Quorum Sensing Regulation of Anthranilate Metabolism in Pseudomonas aeruginosa, Mol. Cells, 32: 57-65, 2011

18. Rui Jiang, Bing Zhang, Kenji Kurokawa, Young-In So, Eun-Hye Kim, Hyun Ok Hwang, Joon-Hee Lee, Akiko Shiratsuchi, Jinghai Zhang, Yoshinobu Nakanishi, Hee-Seung Lee, and Bok Luel Lee*. 93-kDa Twin-domain Serine Protease Inhibitor (Serpin) Has a Regulatory Function on the Beetle Toll Proteolytic Signaling Cascade J. Biol. Chem  286 (40): 35087-35095, 2011

19. Kim, Soo-Kyoung, Cheoljin Kim, Jeyong Yoon, and Joon-Hee Lee*. Inhibition of Quorum Sensing and Biofilm Formation by Synthetic Quorum Signal Analogues in Pseudomonas aeruginosa. Korean J. Microbiol. Biotechnol. 39(1): 29-36, 2011

20. Taeyoung Kim, Junil Kang, Joon-Hee Lee, Jeyong Yoon*. Influence of attached bacteria and biofilm on double-layer capacitance during biofilm monitoring by electrochemical impedance spectroscopy. Water Research, 45: 4615-4622, 2011

21. Kang-Mu Lee, Mi Young Yoon, Yongjin Park, Joon-Hee Lee, and Sang Sun Yoon*. Anaerobiosis-Induced Loss of Cytotoxicity Is Due to Inactivation of Quorum Sensing in Pseudomonas aeruginosa. Infection and Immunity, 79(7) : 2792-2800, 2011

22. S-J. Park, H.Y. Chung and J-H. Lee*. Rapid in vivo screening system for anti-oxidant activity using bacterial redox sensor strains. Journal of Applied Microbiology, 108(4): 1217-1225, 2010.

23. Kyung-Ju Jung, Yusang Choi, Changwan Ha, Jeong Hwan Shin, and Joon-Hee Lee*. Analysis of Quorum Sensing-Related Phenotypes of Pseudomonas aeruginosa Clinical IsolatesThe Korean Journal of Microbiology, 46(3):240-247, 2010

24. Hai-Bo Liu, Joon-Hee Lee, Jung Sun Kim, Sunghoon Park*. Inhibitors of the Pseudomonas aeruginosa Quorum-Sensing Regulator, QscR. Biotechnology and Bioengineering, 106(1):119-126, 2010.

25. Cheoljin Kim, Jaeeun Kim, Hyung-Yeon Park, Joon-Hee Lee*, Hee-Jin Park, Chan Kyung Kim, Jeyong Yoon*. Structural understanding of quorum-sensing inhibitors by molecular modeling study in Pseudomonas aeruginosa. Applied Microbiology and Biotechnology, 83: 1095-1103, 2009.

26. Hee-Jin Park, Jee Yeon Kim, Jaeeun Kim, Joon-Hee Lee*, Ji-Sook Hahn, Man Bock Gu, Jeyong Yoon*. Silver-ion-mediated reactive oxygen species generation affecting bactericidal activity. Water Research, 43: 1027-1032, 2009.

27. Joon-Hee Lee*, Kang-Lok Lee, Won-Sik Yeo, Su-Jin Park, and Jung-Hye Roe. SoxRS-Mediated Lipopolysaccharide Modification Enhances Resistance against Multiple Drugs in Escherichia coli. J. Bacteriol., 191(13): 4441-4450, 2009.

28. Jaeeun Kim, Hee-Jin Park, Joon-Hee Lee*, Ji-Sook Hahn, Man Bock Gu, Jeyong Yoon*. Differential effect of chlorine on the oxidative stress generation in dormant and active cells within colony biofilm. Water Research, 43: 5252-5259, 2009.

29. Cheoljin Kim, Jaeeun Kim, Hyung-Yeon Park, Hee-Jin Park, Chan Kyung Kim,Jeyong Yoon*, and Joon-Hee Lee*. Development of Inhibitors against TraR Quorum-Sensing System in Agrobacterium tumefaciens by Molecular Modeling of the Ligand-Receptor Interaction. Molecules and Cells, 28: 447-453, 2009.

30. Hai Bo Liu, Kyong Pyo Koh, Joon Hee Lee, Jung Sun Kim, and Sunghoon park*. Characterization of LasR Protein Involved in Bacterial Quorum Sensing Mechanism of Pseudomonas aeruginosa. Biotechnology and Bioprocess Engineering, 14: 146-154, 2009.

31. Kim, Cheoljin, Hyung-Yeon Park, Jaeeun Kim, Hee-Jin Park, Bon Su Lee, Yusang Choi, Joon-Hee Lee, and Jeyong Yoon*. Solid Phase Synthesis of N-(3-hydroxysulfonyl)-L-homoserine Lactone Derivatives and their Inhibitory Effects on Quorum Sensing Regulation in Vibrio harveyi. Kor. J. Microbiol. Biotechnol., 37(3):248-257, 2009

32. Kim C, Kim J, Park HY, Park HJ, Lee JH, Kim CK, Yoon J*. uranone derivatives as quorum-sensing antagonists of Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol., 80(1):37-47, 2008

33. Oglesby AG, Farrow JM 3rd, Lee JH, Tomaras AP, Greenberg EP, Pesci EC, Vasil ML*. The influence of iron on Pseudomonas aeruginosa physiology: a regulatory link between iron and quorum sensing. J. Biol. Chem., 283(23):15558-15567, 2008

34. Joon-Hee Lee*. Minority report; Diketopiperazines and Pyocyanin as Quorum Sensing Signals in Pseudomonas aeruginosa. Kor. J. Microbiol. 44(2):85-92, 2008

35. Won-Sik Yeo, Joon-Hee Lee, Kyung-Changand Jung-Hye Roe*. IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins. Mol. Microbiol., 61(1):206-218, 2006

36. Yannick Lequette, Joon-Hee Lee, Fouzia Ledgham, Andree Lazdunski, and E. Peter Greenberg*. A Distinct QscR Regulon in the Pseudomonas aeruginosa Quorum Sensing Circuit. J. Bacteriol., 188(9):3365-3370, 2006

37. Joon-Hee Lee, Yannick Lequette, E. Peter Greenberg*. Activity of purified QscR, a Pseudomonas aeruginosaorphan quorum-sensing transcription factor. Mol. Microbiol., 59(2):602-609, 2006

38. Joon-Hee Lee, Won-Sik Yeo, and Jung-Hye Roe*. Induction of the sufA operon encoding Fe-S assembly proteins by superoxide generators and hydrogen peroxide: involvement of OxyR, IHF and an unidentified oxidant-responsive factor. Mol. Microbiol., 51(6):1745-1755, 2004

39. Joon-Hee Lee, Won-Sik Yeo, and Jung-Hye Roe*. Regulation of the sufABCDSE Operon by Fur J. Microbiol., 41(2):109-114, 2003

40. Koo MS, Lee JH, Rah SY, Yeo WS, Lee JW, Lee KL, Koh YS, Kang SO, and Roe JH. A reducing system of the superoxide sensor SoxR in Escherichia coli. EMBO J., 22(11):2614-2622, 2003, Lee JH and Koo MS are equally contributed to this paper.

41. Nam TW, Cho SH, Shin D, Kim JH, Jeong JY, Lee JH, Roe JH, Peterkofsky A, Kang SO, Ryu S, and Seok YJ*. The Escherichia coli glucose transporter enzyme IICB(Glc) recruits the global repressor Mlc. EMBO J., 20(3):491-498, 2001

42. Koh YS, Chung WH, Lee JH, and Roe JH*. The reversed SoxS-binding site upstream of the ribA promoter in Escherichia coli. Mol Gen Genet., 261(2):374-380. 1999

43. Lee, Joon-Hee, and Jung-Hye Roe*. Isolation and characterization of paraquat-inducible promoters from Escherichia coli. J. Microbiol., 35: 277-283, 1997

44. Koh YS, Choih J, Lee JH, and Roe JH*. Regulation of the ribA gene encoding GTP cyclohydrolase II by the soxRS locus in Escherichia coli. Mol Gen Genet., 251(5):591-598, 1996
 

Patents

1. Method of integratedly analyzing a microorganism and a biofi1m chip used for the same (미생물의 통합적 분석 방법 및 이에 사용되는 생물막 칩). 이준희, 신규순, 윤제용, 홍순규, 이종찬, 이홍금. 특허등록 (patent registration: 10-0884935). 2009-02-16.

2. High sensitivity electrode having micro interdigit structure (마이크로 인터디지트 구조를 구비한 고감도 전극). 신규순, 윤제용, 이준희, 조요한, 유귀덕, 김춘수. 특허출원 (patent application: 10-2008-0116459). 2008-11-21.

3. Method of detecting a biofilm (생물막 측정 방법). 윤제용, 김춘수, 박희진, 신규순, 이준희. 특허출원 (patent application: 10-2008-0111063). 2008-11-10

4. Method of integratedly analyzing a microorganism and a biofi1m chip used for the same (미생물의 통합적 분석 방법 및 이에 사용되는 생물막 칩). 신규순, 이준희, 홍순규, 윤제용, 이종찬, 이홍금. 특허출원 (patent application: 10-2007-0108854). 2007-10-29

Textbooks

1. 약품 미생물학 요약 및 연습 (ISBN 978-89-6154-180-0). 대한 약학회 감염미생물-면역약학 분과학회 공저. 라이프사이언스. 2014-09-01

2. 면역학 (Immunology, a short course) (ISBN: 978-89-5881-204-3). 강호영, 권오식, 김병삼, 김평현, 문은이, 이준희, 임선영, 정용석, 정유진, 조영준, 최태진 공역. 월드사이언스. 2012-09-30

3. 약품 미생물학 (ISBN; 978-89-6154-092-6). 강복윤, 강재선, 강창률, 고현정, 곽진환, 김경제, 김동현, 김홍진, 남두현, 민혜영, 송석길, 신혜순, 유진철, 이강만, 이도익, 이동권, 이만형, 이복률, 이종길, 이주영, 이준희, 임미정, 임재윤, 장준, 표석능, 하남주, 한용문, 황광우 (미생물 면역분과회 공저). 라이프사이언스. 2011-03-01

4. 신 종합 미생물학 (ISBN 978-89-5702-162-0). 손은화, 문경호, 김동현, 이만형, 문애리, 한용문, 이준희, 김경제, 하남주, 강창율, 최응칠, 이동권, 표석능, 임미정, 남두현, 임재윤, 이강만, 강복윤, 유진철, 김홍진, 이도익, 황광우, 정경수, 송석길, 이종길 (대한 약학회 미생물 면역 분과회 공저). 신일 출판사. 2008-03-01

Others

1. 이준희*. Dental biofilm에 대하여. Biochemistry and Molecular Biology News. 6:1-5, 2008

2. 이준희*. 생물막 (biofilm)에 대한 최신 연구 동향. Biochemistry and Molecular Biology News. 9:34-39,2007